Chinese name: Isopropyl- β- D-thiopyranogalactoside
Trade name: Isopropyl β- D-1-Thiogalactopyranoside
English abbreviation: IPTG
Product alias: Isopropyl β- D-Thiogalactoside
General CAS: 367-93-1
Product purity: Sigma ≥ 99%, Merck ≥ 98%, INALCO ≥ 99%
Product properties: white or white powder, clear and colorless liquid after being dissolved in water
Molecular formula: C9H18O5S
Molecular weight: 238.30
Storage temperature: cold storage at 2-8 ° C, storage at - 20 ° C after preparation, and storage at room temperature for one month
Main functions: Isopropyl thiogalactoside (IPTG) is a powerful inducer, which is not metabolized by bacteria and is very stable, so it is widely used in laboratories
IPTG Yes β– Activity inducer of galactosidase. Based on this characteristic, when the vector DNA of pUC series (or other vector DNA with lacZ gene) is transformed with lacZ deletion cells as the host, or when the vector DNA of M13 phage is transfected, if X – Gal and IPTG are added to the plate medium β– Galactosidase α– Complementarity, it is convenient to select gene recombinants according to whether white colonies (or plaque) are present. In addition, it can also be used as an expression inducer of expression vectors with lac or tac promoters.